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However, these methods cannot overcome the size limitation, i.e., if the cultured tissue size exceeds a 100-μm order, the tissue undergoes necrosis due to hypoxia. There are various methods to improve oxygen supply, such as culture inserts and rotator culture. Ī technical obstacle for standard culture systems of three-dimensional tissue structures is the lack of microcirculation. Recent advancements in stem cell biology have enabled the generation of small tissue structures from a single cell, which is called an organoid, and various organoids have been established. An extensively used tool to understand the mechanism of pattern formation is an organ culture system in which embryonic tissue is cultured at the air-liquid interface. Multicellular pattern formation has been one of the central issues in developmental biology. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium.
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Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. As a part of SCO Connect Fellowship funded by BBC Performing Arts Fund.A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Worked with primary school children from the Orkeny Isalnds (Papay, Eday, North Ronaldsay, North Walls, Rousay, Shapinsay and Burray Primary Schools), music animateur Stephen Deazley and SCO Connect Musicians. As a part of SCO Connect Fellowship funded by BBC Performing Arts Fund. Worked with primary school children in Edinburgh for composing and performing works inspired by pictures at Scottish National Portrait Gallery, together with music animateur Greg Sinclair, SCO Connect Musicians, and Artists based in Edinburgh. The workshops was led by composer and music animateur John Barber, SCO Connect and Scottish folk musicians. Involved in the composition and song writing projects with the school children at Scottish Borders to commemorate the 500 years anniversary of the Battle of Flodden. We toured 4 additional support needs schools in Aberdeenshire, Scotland, giving 8 school workshops, 4 school performances, and 2 public performances in Woodend Barn Banchory, all in one week! Here is a lovely short clip of our show (thank you Symon Macintyre!)įantastic working with the school children with additional support needs at Saltersgate School, in Dalkeith, culminating in the performance of Gauche the Cellist with music and shadow puppetry performed by the students of Saltersgate School, Vision Mechanics and the musicians from SCO. Intensive composition workshop with young composers with Red Note Ensemble as a part of Sound Festival in Aberdeen, Scotland.Ī lot of fun working with the school children in the Composition Workshop of ‘Music, Sound and Electronics’ by Lauren Hays and Youth Music Initiative.Ībsolutely wonderful time devising and executing artistic direction and composition workshops for production ‘Gauche the Cellist’ for music and shadow puppetry.
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